Journal: bioRxiv
Article Title: The accumulation of colibactin intermediates does not affect the growth and morphology of pks + Escherichia coli
doi: 10.1101/2020.04.17.047712
Figure Lengend Snippet: Deletion of the ClbP gene in pks + E. coli strain IHE3034. (A) PCR confirmation of clbP deletion. 1kb DNA standars, (2 and 3) PCR Blank (4 and 5) E. coli IHE3034 pks+ (6 to 9) E. coli IHE3034 Δ clbP (10 and 11) E. coli DH10B pks − . B) Giemsa staining of HeLa cell line after 72 hours of post-infection with IHE3034 (pks + ), IHE3034 (Δ clbP ) and DH10B (negative control) E. coli strains at 37 °C and 5 % CO 2 . Bacterial infection was for 4 hours at 37 °C and 5 % CO 2 . All optical microscope images were taken at 40X. C) Growth Curve of IHE3034 (pks + ), IHE3034 (Δ clbP ) and DH10B E. coli strains at 37 °C under constant shaking.
Article Snippet: SEM analysis was performed with the high resolution field emission scanning electron microscope (JEOL JSM-7500F SEM), using 1 kV acceleration voltage for a gentle electron beam.
Techniques: Staining, Infection, Negative Control, Microscopy